A Phylogenetic Index for Cichlid Microsatellite Primers
نویسندگان
چکیده
منابع مشابه
A Phylogenetic Index for Cichlid Microsatellite Primers
Microsatellites abound in most organisms and have proven useful for a range of genetic and genomic studies. Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon. We use PCR amplification, in a 96-well format, to determine the presence and absence of 46 microsatellite loci in 13 cichlid species. At least one primer set amplified a pr...
متن کاملTitle: A Phylogenetic Index of Cichlid Microsatellites
Microsatellites, or short sequence repeats, abound in most organisms and have proven useful for a range of genetic and genomic studies. Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon with varying degrees of success. We use PCR amplification, in a 96 well format, to determine the presence and absence of 46 microsatellite loci i...
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In wild animal populations, the degree of inbreeding differs between species and within species between populations. Because mating with kin often results in inbreeding depression, observed inbreeding is usually regarded to be caused by limited outbreeding opportunities due to demographic factors like small population size or population substructuring. However, theory predicts inclusive benefit...
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PREMISE OF THE STUDY Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. METHODS AND RESULTS We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which...
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ژورنال
عنوان ژورنال: International Journal of Zoology
سال: 2010
ISSN: 1687-8477,1687-8485
DOI: 10.1155/2010/482732